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Author Topic: Damage brain  (Read 5458 times)

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Offline Evelinehoebe

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Damage brain
« on: September 11, 2003, 09:27:30 AM »
Hello,
I work with mousebrains with braintumors, which are treated with genetherapy (virus) and irradiated as well.

Do you have a suggestion for me how to check for brain dammage, caused either by the virus or the irradiation?
I don't have a lot of experience with histochemie.

I found something about a Kluver - Barrera method to stain for myelin, but I also found different protocols for this staining. Which should I use?

Thanks  :)

Damage brain
« on: September 11, 2003, 09:27:30 AM »

Offline susan

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Damage brain
« Reply #1 on: September 13, 2003, 09:34:49 PM »
It depends on what you are looking for. A simple H&E or Nissl staining will give you a general view of the brain structure and morphological changes. You can also use some special stains such as myelin as you mentioned if you are expecting myelin degeneration. To be more specific, you may use immunochemistry method to detect protein/peptide/receptor changes or use apoptosis detection to view if there are any cell death.

In most recently years, neuroscience researchers are using stereological analysis to detect neuronal loss, and this method becomes more important than ever before.

Hope this helps

Susan

Damage brain
« Reply #1 on: September 13, 2003, 09:34:49 PM »