Author Topic: Somatostatin IHC help  (Read 568 times)

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Offline SDashiell

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Somatostatin IHC help
« on: March 05, 2018, 11:42:19 AM »
Has anyone successfully done an immunohistochemical stain against somatostatin? If so, could you kindly share your protocol, including specific primary and secondary antibodies?

Our lab has tried numerous protocol variations to no avail on 40 um mouse brain sections--we either get no signal or very patchy, light cell body staining near the outer edges of the sections. We have been using Millipore MAB354 primary and Invitrogen A11006 secondary.

If anyone could share some insight on staining this pesky protein, it would be very much appreciated!

Below is our latest IHC protocol:

3 x 20 min. wash in PBST
2 h blocking step
48 h Primary incubation at 4C (1:200 in PBST)
3 x 20 min. wash in PBST
4 h secondary incubation (1:500 in PBST)
3 x 20 min. wash in PBS


PBST:
0.1% Triton X-100
in 1x PBS

Blocking solution:
0.1% Triton X-100
5% normal goat serum
0.2% bovine serum albumin
in 1x PBS

Thanks very much!

-Dash

Somatostatin IHC help
« on: March 05, 2018, 11:42:19 AM »