Author Topic: Ph 6:00  (Read 293 times)

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Offline Histolab

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Ph 6:00
« on: June 01, 2018, 09:09:32 AM »
Hi,

I will be perfusing rodent brains using 4% PFA and PBS at PH 6:00, but I am concerned that for IEg staing for C-fos and EGR-1 (Zif) the incubation medium is always used at PH 7.4, is the preparation of the tissue at PH 6.0 going to interfere with the c-fos/Zif ractivity?

Any advice will be appreciated

Ph 6:00
« on: June 01, 2018, 09:09:32 AM »