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Author Topic: Alcian Blue Staining Protocol for Paraffin Sections  (Read 7786 times)

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Offline ihcwor2

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Alcian Blue Staining Protocol for Paraffin Sections
« on: March 12, 2003, 09:46:27 PM »
Alcian Blue Staining Protocol for Paraffin Sections


Solutions and Reagents:

A.   3% Acetic Acid Solution:
             Glacial acetic acid --------------- 3 ml
             Distilled water --------------------- 97 ml

B.   Alcian Blue Solution (pH 2.5):
             Alcian blue, 8GX ----------------- 1 g
             Acetic acid, 3% solution ------- 100 ml

C.   Nuclear Fast Red Solution


Procedure:

1.   Deparaffinize slides and hydrate to distilled water.
2.   Stain in alcian blue solution for 30 minutes.
3.   Wash in running tap water for 2 minutes.
4.   Rinse in distilled water.
5.   Counterstain in nuclear fast red solution for 5 minutes.
6.   Wash in running tap water for 1 minute.
7.   Dehydrate and clear through 95% ethyl alcohol, absolute ethyl alcohol and xylene.
8.   Mount with resinous medium.


Results:

      Strongly acidic sulfated mucosubstances ----------- blue
      Nuclei --------------------------------------------------------- pink to red
      Cytoplasm --------------------------------------------------- pale pink

Alcian Blue Staining Protocol for Paraffin Sections
« on: March 12, 2003, 09:46:27 PM »