Abcam Ad

Author Topic: Black flakes on slide staining  (Read 378 times)

0 Members and 1 Guest are viewing this topic.

Offline EAlberts

  • Newbie
  • *
  • Posts: 1
Black flakes on slide staining
« on: August 07, 2018, 10:00:41 AM »
Hi I am fairly new to IHC,

I have done some staining recently which has come out quite nicely, but recently I have had some that come out with black flakes on the top - does anyone know what this might be? Is it DAB precipitate or contamination from contaminated PBS?

Thanks,

Ellie

Black flakes on slide staining
« on: August 07, 2018, 10:00:41 AM »

Offline KRyan1800

  • Newbie
  • *
  • Posts: 1
Re: Black flakes on slide staining
« Reply #1 on: November 13, 2018, 02:22:56 PM »
What tissue are you staining? I think I may have your exact problem with my small intestine tissue. I was told by a pathologist that you tend to see those black flakes with mucosal tissue like intestine or salivary glands because they are sticky. She said it was crystallized hematoxylin and that filtering it with a paper towel before should do the trick. I have not had that problem since.

Best,

Ryan

Re: Black flakes on slide staining
« Reply #1 on: November 13, 2018, 02:22:56 PM »