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Author Topic: Enzyme pretreatment with protease  (Read 5000 times)

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Offline n_bjorkhammer

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Enzyme pretreatment with protease
« on: January 05, 2005, 07:49:07 AM »
Currently using a Biogenex Protease solution which is, according to manufacturer, stable for 3-5 days. Our problem is that due to a low number of immuno each week, we don't use all protease (5ml) before it expires... Is it possible to freeze this solution in say 1ml vials and defrost them when needed? This web site also provide a protease protocol. Is it proven to work just as good as any other protease digistion system?

Thanks

Niclas
Niclas Björkhammer, MSc
Technologist II - anatomical Pathology and Morgue
Department of Pathology
Sidra Medical and Research Center
Doha, Qatar

Enzyme pretreatment with protease
« on: January 05, 2005, 07:49:07 AM »

Offline richard03

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Enzyme pretreatment with protease
« Reply #1 on: January 05, 2005, 10:00:34 PM »
Just used a few times of the protease protocol on this site and it works well for VWF staining. There is no problem to freeze and store at -20 C for future use but try to avoid repeat freeze-thaw.

Never compared this protocol with commercial reagents. But I think it may work better if you make the solution fresh.

Richard

Enzyme pretreatment with protease
« Reply #1 on: January 05, 2005, 10:00:34 PM »