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Author Topic: Staining with a recombinant protein  (Read 1546 times)

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Offline frabarone

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Staining with a recombinant protein
« on: January 14, 2005, 11:26:59 AM »
Hi,
I'm try to detect on paraffin sections the presence of plasmacells displaying a specific antibody that recognize a recombinant protein that I've got.
This method is working on frozen section . Does anyone have experience about how to make it work on paraffin?
How should I treat the samples without affeting the antibody structure? Should I make a retrieval or just dewax them?
Thanks

Staining with a recombinant protein
« on: January 14, 2005, 11:26:59 AM »