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Author Topic: Any TUNEL assays that use probe other than flourescein???  (Read 8754 times)

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Offline Chakobsa

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Any TUNEL assays that use probe other than flourescein???
« on: January 24, 2005, 03:19:54 PM »
Hello all,
         I just began working with the Roche InSitu Cell Death Detection Kit.  Does anyone know of any TUNEL assays that use a probe other than flourescein?

  :mrgreen:
Catherine

Any TUNEL assays that use probe other than flourescein???
« on: January 24, 2005, 03:19:54 PM »

Offline richard03

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Any TUNEL assays that use probe other than flourescein???
« Reply #1 on: January 24, 2005, 07:52:26 PM »
Check the following protocol for TUNEL assay using enzyme method. You need to purchase Terminal transferase and Biotin-16-dUTP that are also from Roche.

http://www.ihcworld.com/_protocols/apoptosis/tunel_enzyme.htm

Offline Chakobsa

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Any TUNEL assays that use probe other than flourescein???
« Reply #2 on: January 24, 2005, 07:59:59 PM »
Ah, thanks.  I had seen this protocol earlier in the day actually.  I have very little background in the work that I'm doing (in biology in general, to be perfectly honest!), so I wasn't sure about that protocol.  I think we were hoping to find another kit like the InSitu one, but this looks like it could be a good alternative.  Will surely show it to my boss though. Thanks much.

Catherine

Offline Chakobsa

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Unsure about the Biotin
« Reply #3 on: January 25, 2005, 06:31:24 PM »
Looking at the Biotin-16-dUTP protocol, I realized that it's not what I'm actually looking for (at least, I don't think so).  So, what I was hoping to find was a TUNEL assay that uses a flourescent probe- just not flourescein.  We're analyzing by LSC.  Any ideas?
Or am I completely mis-understanding the Biotin thang?!

Catherine

Offline ImmunoNYC

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Any TUNEL assays that use probe other than flourescein???
« Reply #4 on: June 17, 2005, 01:46:15 PM »
Try Apotag from Promega.

Any TUNEL assays that use probe other than flourescein???
« Reply #4 on: June 17, 2005, 01:46:15 PM »