Author Topic: how to get rid of the non-specific staining on mouse spleen  (Read 3101 times)

0 Members and 1 Guest are viewing this topic.

03704462

  • Guest
how to get rid of the non-specific staining on mouse spleen
« on: January 28, 2005, 06:35:28 AM »
Hi, I am doing gamma-H2AX staining on mouse tissue by using M.O.M.(MOUSE ON MOUSE) kit. It works fine in most tissue, but for spleen I got high background even applied Biotin-avidin blocking buffer. How can I get rid of them?Thank you very much.

how to get rid of the non-specific staining on mouse spleen
« on: January 28, 2005, 06:35:28 AM »

Offline REmerson

  • SuperTech
  • ****
  • Posts: 37
how to get rid of the non-specific staining on mouse spleen
« Reply #1 on: February 01, 2005, 09:40:33 AM »
You can try additional blocking of endogenous alkaline phosphatase.
Try using 15% acetic acid at 4 degrees for 1 minute.  I do this step prior to by normal blocking.

03704462

  • Guest
how to get rid of the non-specific staining on mouse spleen
« Reply #2 on: February 02, 2005, 06:38:20 AM »
Thank you for response. I will try it, and another question is what solutions or reagents should I use to dilute citrate acid?

how to get rid of the non-specific staining on mouse spleen
« Reply #2 on: February 02, 2005, 06:38:20 AM »