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Author Topic: The best embedding medium for immunohistochemistry  (Read 8225 times)

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Offline bertie1

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The best embedding medium for immunohistochemistry
« on: February 04, 2005, 09:39:06 AM »
Hi guys,

I have a quick question. I want to do immunohistochemistry on thin sections (2-10 micron) and want to know which medium is the best for the job. I really want to preserve as much of the cellular and even subcellular structure as possible and without much tissue shrinkage. I have been looking into methacrylates but there are so many that I'm unsure as to which would be the most suitable?

Are there any pitfalls with using methacrylates over paraffin? One of the advantages I can see with some methacrylates is that TEM is also possible. But I figure that removing the resin is not the nicest.

Thanks,

Bertie

The best embedding medium for immunohistochemistry
« on: February 04, 2005, 09:39:06 AM »

Offline excalibur

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The best embedding medium for immunohistochemistry
« Reply #1 on: February 04, 2005, 05:31:31 PM »
Try glycolmethacrylate. Polysciences website has some very good descriptions of their different resin kits and the use of each.
Make sure your resin does not generate too much heat while polymerizing. This can destroy antigens.
Ultramicrotomes for sectioning are very expensive. Then you will need glass knives for thick sections. And a glass knife maker to make the knives, plus accessories to attach the little water baths to the knives.

Good luck.
Paula Keene Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
5830 N Blue Lake Dr.
Norman, OK 73069
405-759-3959
www.excaliburpathology.com

Offline ImmunoNYC

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The best embedding medium for immunohistochemistry
« Reply #2 on: June 17, 2005, 01:48:43 PM »
I get great immunostaining on 4um paraffin sections with near perfect preservation of overall morpohology.

The best embedding medium for immunohistochemistry
« Reply #2 on: June 17, 2005, 01:48:43 PM »