Abcam Ad

Author Topic: can one 'strip' paraffin sections for repeated IF usage?  (Read 3040 times)

0 Members and 1 Guest are viewing this topic.

Offline jacqueline

  • Newbie
  • *
  • Posts: 1
can one 'strip' paraffin sections for repeated IF usage?
« on: February 11, 2005, 06:12:08 PM »
Hi,

Having paraffin sections of relatively unique specimens, and limited in fluorescence detection to red (e.g. texas-red) and green (e.g FITC) I wonder if any of you could provide me with protocols of stripping the slides of the first set of antibodies, so that they can be used for another set of antibodies in immunofluorescence. What will do the trick, heat, detergent, other? How many times can sections be stripped? Is there a difference depending on fixation of the specimens (paraformaldehyde, Methanol, methanol-based Carnoys, other?)?

Thanks very much in advance,
Jacqueline

can one 'strip' paraffin sections for repeated IF usage?
« on: February 11, 2005, 06:12:08 PM »