Author Topic: staining of psammomys pancreas with rabbit anti HNE (Read 11269 times)

merav fraenkel · « **on:** February 13, 2005, 05:03:20 AM »

I followed the protocol published in this website to stain pancreatic sections of diabetic psammomys obesus without success. I tried overnight incubation at 4 degrees and also tried using a florescent secondary AB withut succuss either.
Does anyone have any further suggestions?
Merav Fraenkel

« **on:** February 13, 2005, 05:03:20 AM »

excalibur · « **Reply #1 on:** February 13, 2005, 08:01:25 AM »

Are your sections frozens or paraffin?

richard03 · « **Reply #2 on:** February 13, 2005, 10:02:07 AM »

The antibody may not have cross reactivity with human tissue.

Richard

merav fraenkel · « **Reply #3 on:** February 14, 2005, 12:51:00 AM »

Quote from: "excalibur"

Are your sections frozens or paraffin?

They are paraffin embedded
Merav

excalibur · « **Reply #4 on:** February 14, 2005, 08:43:05 AM »

You may need to do an antigen retrieval procedure such as boiling in citrate buffer to unmask your antigen.

merav fraenkel · « **Reply #5 on:** February 15, 2005, 01:36:04 AM »

Quote from: "excalibur"

You may need to do an antigen retrieval procedure such as boiling in citrate buffer to unmask your antigen.

I did that already although this is not a nuclear Ag

« **Reply #5 on:** February 15, 2005, 01:36:04 AM »

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Author Topic: staining of psammomys pancreas with rabbit anti HNE (Read 11269 times)