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Author Topic: GFP and PFA  (Read 3170 times)

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Offline landras

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GFP and PFA
« on: February 24, 2005, 02:55:17 PM »
Hi, I have a transgenic mouse that express GFP (mPer1::GFP) and when i did the immuno using a antibody against mPER1 on this transgenics brains I found that GFP pattern didn't match the PER1 pattern. I need to know if someone had experience using animals that express GFP, which fixative are you using? I think that the fixative (PFA) coud be the problem in my case. thank you

GFP and PFA
« on: February 24, 2005, 02:55:17 PM »