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Author Topic: where to begin  (Read 1835 times)

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Offline ljkuconn

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where to begin
« on: April 19, 2005, 01:15:07 PM »
I have received a rabbit polyclonal antiobody made against a peptide of interest. It has never been used for IHC, only for Westerns at 1:5000.

I am supposed to use it for IHC on slides of tissue from paraffin embedded blocks.

I have no idea what type of protocol to use or where to begin. Any suggestion??

Thanks.
ljkuconn

where to begin
« on: April 19, 2005, 01:15:07 PM »

Offline richard03

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where to begin
« Reply #1 on: April 19, 2005, 02:38:03 PM »
The following method can be your reference and starting point:

http://www.ihcworld.com/_protocols/general_IHC/immunoenzyme_pod.htm

where to begin
« Reply #1 on: April 19, 2005, 02:38:03 PM »