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Author Topic: Citrate antigen retrievel  (Read 7175 times)

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Offline lecor

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Citrate antigen retrievel
« on: April 27, 2005, 11:08:25 AM »
Hi
I am staining cruciate ligaments. The antigen retrievel I'm using is the citrate-microwave method. The problem I have is that within 5 minutes in the solution, most of the tissue detaches from the slides. Is there anyone out there who may have any ideas on how i can prevent this from happening.
Thanks

Neal
eal

Citrate antigen retrievel
« on: April 27, 2005, 11:08:25 AM »

Offline excalibur

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Citrate antigen retrievel
« Reply #1 on: April 27, 2005, 02:07:58 PM »
Are you using silane or Plus slides? You will need to use coated slides and not just adhesive in the water bath.
Paula Keene Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
5830 N Blue Lake Dr.
Norman, OK 73069
405-759-3959
www.excaliburpathology.com

Offline lecor

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Citrate antigen retrievel
« Reply #2 on: April 27, 2005, 04:10:40 PM »
I'm using super frosted plus slides
eal

Offline excalibur

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Citrate antigen retrievel
« Reply #3 on: April 27, 2005, 05:05:59 PM »
Great. Are you letting them dry completely before deparaffinizing?

They should air dry for a couple of hours and then placed in a 58 C oven for about 30 minutes. The oven is not really to melt the paraffin as much as it is to dry. The oven should be used right before deparaffinzing or they can reabsorb atmospheric water. If you have slides that have been cut awhile, always place them in the oven first.
Paula Keene Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
5830 N Blue Lake Dr.
Norman, OK 73069
405-759-3959
www.excaliburpathology.com

Offline lecor

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Citrate antigen retrievel
« Reply #4 on: April 27, 2005, 06:03:59 PM »
I've been placing them in an oven set at 60 degrees for 30 mins prior to deparafinization
eal

Offline excalibur

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Citrate antigen retrievel
« Reply #5 on: April 27, 2005, 06:09:06 PM »
Then you may have a bad lot of slides. I have had that happen.

Also, don't leave the slide in the water bath very long to pick up the section. Get your sections ready and then quickly pick them up.
Paula Keene Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
5830 N Blue Lake Dr.
Norman, OK 73069
405-759-3959
www.excaliburpathology.com

Offline bless

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Citrate antigen retrievel
« Reply #6 on: July 05, 2005, 06:06:35 AM »
hi there.

we have had that problem before. especially, we noticed in tissues that are not properly fixed.

if u want to try, we are using plain slides that are coated with histogrip(zymed).we have excellent adherence with this even if "baking" of slides exceeds the required time. just be sure to properly wash the slides with dist water when coating. good luck. :)

Citrate antigen retrievel
« Reply #6 on: July 05, 2005, 06:06:35 AM »