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Author Topic: Beta-galactosidase mononclonal  (Read 5658 times)

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Offline bn

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Beta-galactosidase mononclonal
« on: June 20, 2005, 01:55:38 PM »
Does anyone know of a monoclonal antibody to Beta-galactosidase that works for formaldehyde fixed, paraffin embedded tissue? I want to detect beta-gal in embryonic mouse sections and although I have a good rabbit polyclonal to beta-gal, I need a mouse monoclonal so I can do double labelling with other rabbit antibodies. Alternatively, does anyone have a protocol for double immunofluorescence with two rabbit primary antibodies?
Many thanks in advance.

Beta-galactosidase mononclonal
« on: June 20, 2005, 01:55:38 PM »

Offline richard03

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Beta-galactosidase mononclonal
« Reply #1 on: June 22, 2005, 08:33:04 PM »
If you use mouse monoclonal on mouse tissue, it will produce heavy background, especially for embryonic tissues.

I am confused. You are looking for monoclonal beta-gal and double label with rabbit b-gal?

If you use two primary from same species, you should do sequential method, meaning one after another.

Here is a protocol for beta-gal staining

http://www.ihcworld.com/_protocols/antibody_protocols/b-gal_abcam.htm

Richard

Beta-galactosidase mononclonal
« Reply #1 on: June 22, 2005, 08:33:04 PM »