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Author Topic: my antigen retreival didnt work!  (Read 5340 times)

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Offline aygroig

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my antigen retreival didnt work!
« on: June 13, 2005, 12:17:14 PM »
I m working with immunohistochemestry for the first time, i had some problem with PDX 1 antibody, i mean i used paraffined tissue  but afther antigen retreival ( sodium citrate 10mM ph 6 , 95 C) and staning with alexa 488, i saw a lot of background and no specific staning!!
What can i do?
Sorry for my english!

my antigen retreival didnt work!
« on: June 13, 2005, 12:17:14 PM »

Offline richard03

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my antigen retreival didnt work!
« Reply #1 on: June 22, 2005, 08:58:01 PM »
The background maybe due to autofluorescence. Check here for more information:

http://www.ihcworld.com/autofluorescence.htm

Richard

Offline aygroig

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my antigen retreival didnt work!
« Reply #2 on: June 24, 2005, 03:25:22 PM »
:D thank you! i ll try! and i ll tell you the results :wink:

my antigen retreival didnt work!
« Reply #2 on: June 24, 2005, 03:25:22 PM »