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Author Topic: Mouse Mammary Tumor Lung Metastasis Markers?  (Read 9877 times)

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Offline Jennybean

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Mouse Mammary Tumor Lung Metastasis Markers?
« on: July 13, 2005, 03:53:45 PM »
I'm an IHC newbie and I'm searching for a marker and/or protocol that I can use to show that lung metastases arise from mammary tumors in B-catenin transgenic mice. Anyone have any ideas?

Mouse Mammary Tumor Lung Metastasis Markers?
« on: July 13, 2005, 03:53:45 PM »

Offline excalibur

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Mouse Mammary Tumor Lung Metastasis Markers?
« Reply #1 on: July 14, 2005, 01:02:30 PM »
Depends on what lung tumor you are looking for. Please be more specific.
Paula Keene Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
5830 N Blue Lake Dr.
Norman, OK 73069
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Offline Jennybean

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Mouse Mammary Tumor Lung Metastasis Markers?
« Reply #2 on: July 14, 2005, 02:26:42 PM »
Hi Paula- Thanks for the quick response! I think that all the primary mammary tumors are adenocarcinomas. I would like to try to show through IHC that the observed lung tumors are metastases from these primary tumors. I haven't been able to find a suitable mammary or mammary tumor marker to test! Please let me know if you have any ideas. Thanks!!!

Offline excalibur

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Mouse Mammary Tumor Lung Metastasis Markers?
« Reply #3 on: July 14, 2005, 02:46:28 PM »
Biogenex has an antibody specific for breast adenocarcinoma.

http://www.biogenex.com/antibody.php?show=000024

Unfortunately it is created in mice and you would need a Mouse on mouse kit.

Try biocompare.com and do a search for breast adenocarcinoma created in rabbit or some other species.

ER/PR would also show that it is breast tissue.
Paula Keene Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
5830 N Blue Lake Dr.
Norman, OK 73069
405-759-3959
www.excaliburpathology.com

Offline ole

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Mouse Mammary Tumor Lung Metastasis Markers?
« Reply #4 on: July 14, 2005, 03:15:29 PM »
hi

There are also an antibody called mammaglobin. I dont know if some produce it yet. Think i saw it on dakocytomation web site (news) a while ago, think they got a license to produce it.

Ole

Offline ImmunoNYC

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Mouse Mammary Tumor Lung Metastasis Markers?
« Reply #5 on: July 17, 2005, 05:39:14 PM »
Are these mouse tumors or human tumors grown in these transgenic mice?

I would recommend for starters you find a cytokeratin antibody. I know of several good ones for human breast carcinoma tumors. Not sure about for mouse but you can look around for anti-mouse cytokeratin antibodies. I am sure they are available somewhere (for example I think DAKO has a wide spectrum one which publications have used with success).

Offline richard03

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Re: Mouse Mammary Tumor Lung Metastasis Markers?
« Reply #6 on: September 27, 2007, 10:31:43 PM »
Here is a full list of breast cancer markers. Hope this is helpful.

http://www.antibodybeyond.com/reviews/tumor-markers/breast-cancer-marker.htm

Re: Mouse Mammary Tumor Lung Metastasis Markers?
« Reply #6 on: September 27, 2007, 10:31:43 PM »