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Author Topic: tissue processor reagent turnover  (Read 7168 times)

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Offline pweston

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tissue processor reagent turnover
« on: July 25, 2005, 10:59:10 AM »
I have begun using a new closed paraffin  tissue processor for research purposes.  There have not been many tissue samples per run.  Usually around 50 in a processor that can hold up to 300.  These runs are infrequent, usually once a week or less.  

(1)-I am not sure how often to rotate reagents.  Should I go with number of runs or number of samples?  How many runs/samples before I rotate reagents?  I don't want to waste chemicals, but also do not want inferior embedding.  The manual for the unit is not clear on this.

(2)-Is the purge cycle affected by batch size, or is there a fixed number of runs before changing the purge alcohol and xylene?    

Thank you.

tissue processor reagent turnover
« on: July 25, 2005, 10:59:10 AM »

Offline excalibur

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tissue processor reagent turnover
« Reply #1 on: July 27, 2005, 01:19:47 PM »
Go by the number of samples processed. The purge cycle solutions will still need to be changed after every 5 runs or so because you still need to clean the paraffin to make it ready for the next run.

With a low volume of specimens you may lose more solution to evaporation than contamination. Just check the levels before every run.
Paula Keene Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
5830 N Blue Lake Dr.
Norman, OK 73069
405-759-3959
www.excaliburpathology.com

tissue processor reagent turnover
« Reply #1 on: July 27, 2005, 01:19:47 PM »