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Special Stain Protocols
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Special Stain Protocols
Scott’s Tap Water Substitute Protocol
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Scotts Tap Water Substitute is a bluing reagent for histology and
Ziehl Neelsen Staining Protocol
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Prepared by ROY ELLIS IMVS Division of Pathology The Queen
X-Gal Staining Protocol for beta-Galactosidase
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Description: This method is used for the detection of β-galactosidase on formalin-fixed, frozen
Modification of Warthin-Starry Silver Technique for the Demonstration of Bacillus Piliformis
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By: Robert Dionne, Histotech Veteran’s Affaire Medical Center White River
Verhoeff-Van Gieson (VVG) Staining Protocol for Elastic Fibers
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Description: This method is used for identifying elastic fibers in tissues
von Kossa and Alcian Blue Staining Protocol
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Description: This technique is for demonstrating deposits of calcium or calcium
von Kossa Staining Protocol for Calcium
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Prepared by ROY ELLIS IMVS Division of Pathology The Queen
von Kossa Staining Protocol for Calcium
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Description: This technique is for demonstrating deposits of calcium or calcium
van Gieson Staining Protocol
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Prepared by ROY ELLIS IMVS Division of Pathology The Queen
Toluidine Blue Staining Protocol for Mast Cells
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Description: Mast cells are found in the connective tissue and their
Southgate’s Mucicarmine Staining Protocol
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Prepared by ROY ELLIS IMVS Division of Pathology The Queen
Sirius Red Staining Protocol for Collagen
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John A. Kiernan Department of Anatomy & Cell Biology, The
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