Histology FAQ

Staining, Histochemistry and Histotechnology

(Frequently Asked Questions)

 

Dr. John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London, Canada

 

 

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FAQ Home > Processing, Decalcifying, Embedding

• Solvent to replace xylene AND alcohols

• 2-butoxyethanol ("Clereum") dehydrating or clearing agent

• Decalcification: Acid or EDTA?

• Testing for completeness of decalcification

• Fatty specimens: Processing into paraffin

• Polymethyl methacrylate embedding for bone

• Mold release spray

• Paraffin processing of skin

• Cryoprotection of specimens

• Cutting sections of toe or finger nails

• Paraffin wax: crystals, additives and cutting

• Xylene substitutes: what are they?

• Test for water in used absolute alcohol

• Molecular sieves for making anhydrous solvent