Histology FAQ

Staining, Histochemistry and Histotechnology

(Frequently Asked Questions)

Dr. John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London, Canada

FAQ Home > Sectioning, Slide Adhesive, Mounting

Thick paraffin sections

Question.

I need a method for cutting near perfect 50 micron sections of paraffin processed tissue. They are not only difficult to cut, but will not stay on the slides! Please advise.

Answer.

We use the plus slides and put about 25 drops of Elmer's school glue in the waterbath. This combination works VERY well for us.

Sarah Ann Christo
(schristo[AT]cvm.tamu.edu)