Immunohistochemistry

Frequently Asked Questions (FAQ)

 

Dr. Giorgio Cattoretti
Associate Professor of Clinical Pathology
Institute for Cancer Genetics, Columbia University
New York, USA

 

 

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Table of Contents

Got a good manual at hand? Frozen or paraffin sections? Immunohistochemistry (IHC) or immunofluorescence (IF)? Which is the most sensitive method? IF or IHC? Alkaline Phospatase (AP) or Peroxidase (HRP)? Which is the most sensitive, HRP or AP? Do I need detergent to permeabilize the cells? For how long should I incubate a primary antibody? How stable are the diluted antibodies? Do I need to freeze my antibody aliquots?

 

 

We are very grateful for Dr. Giorgio Cattoretti generous contribution of this Immunohistochemistry FAQ article. For more information and latest updates, please visit his website at http://icg.cpmc.columbia.edu/cattoretti/Protocol/Main.html