Technical Bulletin for Histotechnology




Vol. XXXVII, No. 1, May 2004 (PDF)


  • Decalcified Bone Sections in Surgically Induced Osteoarthritis in Rat.
    Amy Rutledge, BS, HTL(ASCP), Elizabeth Chlipala, BS, HTL(ASCP), Premier Histology Laboratory, LLC, University of Colorado, Boulder, CO, lizchlipala@premierhistology.com. May 2004;XXXVII(1):1.

  • Preparation and Snap Freezing of Murine Tissues for Research Immunohistochemistry and Routine Hematoxylin & Eosin Staining.
    Gayle Callis, MT, HTL(ASCP)HT, Veterinary Molecular Biology, Montana State University, Bozeman, MT. May 2004;XXXVII(1):4.

  • A Novel Technique to Embed Tissues for Frozen Section Cryotomy.
    Carlette Mitchell Geddis, BS, HTL(ASCP), Medical University of South Carolina, Charleston, SC. May 2004;XXXVII(1):7.

  • Debunking an Urban Legend in Bone and Cartilage Histotechnique: Safranin O Fast Green Will Stain Proteoglycans After EDTA Decalcification.
    Robert A. Skinner, Dept. of Orthopaedic Research, University of Arkansas for Medical Sciences, Little Rock, AR, Skinnerroberta@uams.edu. May 2004;XXXVII(1):10.

  • Immunohistochemical Staining Techniques to Demonstrate Treponema pallidum Spirochetes.
    Hazel Dalton, BA, HT, QIHC (ASCP), Lydia Shanks, HT (ASCP), Roxanne Walden, BS, HTL, QIHC (ASCP), University of Texas, M.D. Anderson Medical Center, Houston, TX. May 2004;XXXVII(1):14.

Vol. XXXVII, No. 2, December 2004 (PDF)


  • A Brief Review of Alzheimer’s Disease by Using Conventional Silver Staining Procedures.
    Alicia A. Zuniga, CLS, HTL (ASCP); Patricia Gonzalez, BS, HT; Antonio Fernandez, MD. School of Natural and Health Sciences, Barry University, Miami Shores, FL. December 2004;XXXVII(2):21.

  • Combined UCHL-1/PASH Staining in the Diagnosis of Renal Transplant Rejection.
    Ranjitha Veerappan, MD; Lou Ellen Miller, HT (ASCP); Piyush Joshi, MD; George A. Youngberg, MD. Pathology Department, East Tennessee State University, Johnson City, TN. December 2004;XXXVII(2):25.

  • Cryomicrotomy of Murine Tissues for Research Immunohistochemistry and Routine Hematoxylin and Eosin Staining Part II.
    Gayle M. Callis, MT, HT, HTL(ASCP). Veterinary Molecular Biology, Montana State University, Bozeman, MT. December 2004;XXXVII(2):29.

  • Alcian Blue-H&E-Metanil Yellow Stain for Diagnosing Barrett’s Esophagus.
    Wendi Edgett, BS, HTL (ASCP). Genesys Regional Medical Center, Health Park Campus, Grand Blanc, MI. December 2004;XXXVII(2):35. 

  • Double Embedding: Double the Trouble?
    M. Reid, MLT; C. Goodwin, MLT. Department of Pathology & Laboratory Medicine, Mount Sinai Hospital, University of Toronto, Canada. December 2004;XXXVII(2):39.