Description: Ueyama et al. (1990) assigned the ACTSA gene to chromosome 10 by Southern blot analysis of DNAs from 18 rodent-human somatic cell hybrids. Regional mapping by in situ hybridization localized the gene to 10q22-q24.Assignment of the vascular smooth muscle actin gene ACTSA to human chromosome. Smooth muscle alpha-actin gene requires two E-boxes for proper expression in vivo and is a target of class I basic helix-loop-helix proteins.
Primary Antibody
Name: alpha-SMA IHC Antibody |
Clone: Mouse Monoclonal |
Supplier: IHC World |
Catalog Number: IW-MA1106 |
Dilution: Ready to Use |
Incubation Time/Temp: 60 min/room temperature |
Device: Pretreatment is not required. |
Buffer/pH value: N/A |
Heat/Cool Temperature: N/A |
Heat/Cool Time: N/As |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Membrane, cytoplasmic |
Images: Search image |
Species Reactivity: Human, mouse, rat |
Fixation: Formalin fixed paraffin embedded sections. |
Positive Control: Smooth muscle, colon, skin, human tonsil. |
Negative Control: Omit primary antibody, isotype control, absorption control |
Blocking: Normal serum blocking is not needed for this RTU antibody; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
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