Description: Adrenocorticotrophic hormone (ACTH) is a peptide hormone produced by the anterior pituitary gland that stimulates the adrenal cortex. It stimulates the adrenal cortex to secrete glucocorticoid hormones, which help synthesise glucose, catabolise proteins, mobilise free fatty acids and inhibit inflammation in allergic responses. The hormone comprises a long chain of amino acids that share a high degree of homology between humans, cows, pigs and sheep.
Primary Antibody
Name: Adrenocorticotrophic Hormone |
Clone: 56, Mouse Anti-Human |
Supplier: Novocastra Labs |
Catalog Number: NCL-ACTH |
Dilution: 1:100 - 1:200 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60 min/room temperature |
Device: Antigen retrieval is not needed |
Buffer/pH value: N/A |
Heat/Cool Temperature: N/A |
Heat/Cool Time: N/A |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Cytoplasmic |
Images: Search image |
Tissue Type: Pituitary gland |
Fixation: Formalin fixed paraffin secitons or acetone fixed frozen sections |
Positive Control: Pituitary gland |
Negative Control: Omit primary antibody, isotype control, absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
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