Description: This antibody is specific for the single isoform of alpha-cardiac muscle actins. It reacts with a-skeletal and a-cardiac muscle actins. It does not react with smooth muscle tissue.
Primary Antibody
Name: Actin (alpha-Sarcomeric) |
Clone: 5C5, Mouse |
Supplier: Sigma-Aldrich |
Catalog Number: A2172 |
Dilution: 1:1000 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60 min/room temperature |
Device: Antigen retrieval is not needed. |
Buffer/pH value: Citrate buffer reduce staining intensity, NOT enhance! |
Heat/Cool Temperature: N/A |
Heat/Cool Time: N/A |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Cytoplasmic |
Images: Search image |
Tissue Type: Rat cardiac muscle |
Fixation: Formalin fixed paraffin secitons or acetone fixed frozen sections |
Positive Control: Cardiac or skeletal muscle. Rat cardiac muscle works well. |
Negative Control: Omit primary antibody, isotype control, absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
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