Description: The antibody (also known as anti-alpha-Sm-1) is specific for the single isoform of alpha-smooth muscle actin. It reacts specifically witha-smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections.
Primary Antibody
Name: Mouse Anti-Actin, alpha Smooth Muscle |
Clone: 1A4, Mouse monoclonal |
Supplier: Sigma |
Catalog Number: A2547 |
Dilution: 1:1000 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60 min/room temperature |
Device: Antigen retrieval is not needed |
Buffer/pH value: N/A |
Heat/Cool Temperature: N/A |
Heat/Cool Time: N/A |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Membrane/Cytoplasmic |
Images: Search image |
Species Activity: Human, mouse, rat, bovine, chicken, frog, goat, guinea pig, rabbit, dog, sheep, snake. |
Tissue Type: Smooth muscle, colon, skin |
Fixation: Formalin-fixed paraffin sections or acetone fixed frozen sections as well as cell smears. |
Positive Control: Smooth muscle, colon, small intestine, tongue, skin |
Negative Control: Omit primary antibody, isotype control, absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
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