Description: This antibody reacts with the muscle actin on formalin fixed paraffin embedded sections or frozen sections.
Primary Antibody
Name: Mouse Anti-Human Actin, Muscle |
Clone: HHF35 |
Supplier: Dako |
Catalog Number: M0635 |
Dilution: 1:200 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60 min/room temperature |
Device: Antigen retrieval is not needed |
Buffer/pH value: N/A |
Heat/Cool Temperature: N/A |
Heat/Cool Time: N/A |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Membrane/Cytoplasmic |
Images: Search image |
Species Reactivity: Human |
Fixation: Formalin-fixed paraffin sections or acetone fixed frozen sections |
Positive Control: Skeletal muscle, tongue, leg muscle |
Negative Control: Omit primary antibody, isotype control, absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
There is no need to perform antigen retrieval on acetone fixed frozen sections |
References: