Description: Alpha-1-Antichymotrypsin (ACT) is an early-stage acute phase protein and a member of the serine proteinase inhibitor or serpin superfamily. The precise role of ACT is uncertain but it is thought to act as an anti-inflammatory agent inhibiting chymotrypsin, cathepsin G, mast cell chymase, neutrophil chemotaxis and superoxide anion production. ACT is synthesized primarily by hepatocytes of the liver. Lower levels of synthesis have also been discovered via immunohistochemical analysis in mast cells, endothelial cells, breast and intestinal epithelial cells. ACT also exists in the brain. Research has shown that it is found in amyloid fibrils, endothelial cells and the cytoplasm of astroglial cells in certain brain abnormalities. Further research has also shown that a major proportion of prostate-specific antigen (PSA) in serum exists complexed to ACT.
Primary Antibody
Name: alpha-1-Antichymotrypsin Antibody |
Clone: Rabbit Polyclonal |
Supplier: Novocastra Labs |
Catalog Number: NCL-A1ACp |
Dilution: 1:100 - 1:200 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60 min/room temperature |
Device: Antigen retrieval is not needed |
Buffer/pH value: N/A |
Heat/Cool Temperature: N/A |
Heat/Cool Time: N/A |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Cytoplasmic |
Images: Search image |
Tissue Type: Tonsil, placenta |
Fixation: Formalin-fixed paraffin sections, Not effective on frozen sections |
Positive Control: Tonsil, placenta |
Negative Control: Omit primary antibody, isotype control, absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
References:
1. Niki T, Oka T, Shiga J, Machinami R (1995) Kupffer cells in multiple organ failure--their activation as revealed by immunohistochemistry for lysozyme, alpha 1-antichymotrypsin, and lectins. Gen Diagn Pathol. 141(1):21-7 PubMed Abstract
2. Uchida K, Kuroki K, Yoshino T, Yamaguchi R,
Tateyama S. (1997)Immunohistochemical study of constituents other
than beta-protein in canine senile plaques and cerebral amyloid
angiopathy.
Acta Neuropathol (Berl). 93(3):277-84.
PubMed Abstract