Description: Amylin is a pancreatic islet peptide with a role in the maintenance of glucose homeostasis. It is predominantly found in the beta cells of the pancreas and to a lesser extent in the gastrointestinal tract and nervous system. Amylin appears to work with insulin to regulate plasma/glucose concentrations in the bloodstream. Two fragments of amylin have been identified in vivo. One fragment contains residues 17 to 37 of human amylin and the other contains residues 24 to 37. Amylin (24-37) peptide fragment forms amyloid deposits, however, smaller fragments eg residues 20 to 29, are also capable of generating amyloid.
Primary Antibody
Name: Amylin Peptide Antibody |
Clone: R10/99, Mouse |
Supplier: Novocastra Labs |
Catalog Number: NCL-AMYLIN |
Dilution: 1:50 - 1:100 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60 min/room temperature |
Device: Antigen retrieval not needed |
Buffer/pH value: N/A |
Heat/Cool Temperature: N/A |
Heat/Cool Time: N/A |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Cytoplasmic and Secreted |
Images: Search image |
Tissue Type: Pancreas |
Fixation: Formalin-fixed paraffin sections |
Positive Control: Pancreas |
Negative Control: Omit primary antibody, isotype control, absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
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