Description: Beta galactosidase is a a metalloenzyme that that splits lactose into glucose and galactose and is coded by a gene (lac z) in the lac operon of Escherichia coli.
Primary Antibody
Name: Beta galactosidase Antibody |
Clone: Rabbit polyclonal |
Supplier: Abcam |
Catalog Number: ab616 |
Dilution: 1:500 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60min/room temperature |
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102) |
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100) |
Heat/Cool Temperature: 95-100 ºC/room temperature |
Heat/Cool Time: 20 minutes/20 minutes |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Nulcear |
Images: Search image |
Tissue Type: Tissue or cells expressing LacZ or Transgenic mice expressing nuclear beta-galactosidase |
Fixation: Formalin-fixed paraffin sections, or paraformaldehyde fixed frozen sections |
Positive Control: Tissue or cells expressing LacZ or Transgenic mice expressing nuclear beta-galactosidase |
Negative Control: Omit primary antibody, isotype control, absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
There is no need to perform antigen retrieval on acetone fixed frozen sections |
References:
The dilution of this antibody varies depending on tissue type, fixation, pretreatment, etc