Description: BAG-1, also called RAP46, is a multifunctional protein that regulates several cellular processes including cell survival, signal transduction and protein folding. BAG-1 is an anti-apoptotic protein capable of interacting with several types of proteins including those of the bcl-2 family, Raf-1, certain tyrosine kinase growth factor receptors and steroid hormone receptors. Alternative translation initiation points in the BAG-1 transcript results in the expression of four protein isoforms known as p50, p46, p33 and p29. The protein is expressed in a number of cell lines and has been demonstrated immunohistochemically to stain both the cytoplasm and nuclei of normal breast tissue.
Primary Antibody
Name: BAG-1 (RAP46 Protein) Antibody |
Clone: 5C5, Mouse Anti-Human |
Supplier: Novocastra Labs |
Catalog Number: NCL-BAG-1 |
Dilution: 1:25 - 1:50 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60min/room temperature |
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102) |
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100) |
Heat/Cool Temperature: 95-100 ºC/room temperature |
Heat/Cool Time: 20 minutes/20 minutes |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Nuclear and cytoplasmic |
Images: Search image |
Tissue Type: Tonsil |
Fixation: Formalin-fixed paraffin sections |
Positive Control: Tonsil |
Negative Control: Omit primary antibody, isotype control, absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
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