Description: In 90% of follicular lymphomas a translocation occurs with juxtaposes the bcl-2 gene at 18q21, to an immunoglobulin gene, with subsequent deregulation of protein synthesis and cell proliferation. The bcl-2 product is considered to act as an inhibitor of apoptosis. For this reason, bcl-2 expression is inhibited in germinal center where apoptosis forms part of the B cell production pathway. This observation has tumed out to have clinical implications. Distribution of follicular hyperplasia form follicular lymphoma is a common problem in histopathology. Reactive follicles show no staining for bcl-2, whereas the cells in neoplastic follicules exhibit membrane and/or cytoplasmic staining.
Primary Antibody
Name: Bcl-2 Oncoprotein Antibody |
Clone: 124, Mouse Anti-Human |
Supplier: Dako |
Catalog Number: M0887 |
Dilution: 1:50 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60min/room temperature |
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102) |
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100) |
Heat/Cool Temperature: 95-100 ºC/room temperature |
Heat/Cool Time: 20 minutes/20 minutes |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Membrane and cytoplasmic |
Images: Search image |
Tissue Type: Tonsil |
Fixation: Formalin-fixed paraffin sections |
Positive Control: Tonsil |
Negative Control: Omit primary antibody, isotype control, absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
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