Description: Bcl6 is a proto-oncogene that encodes Kruppel type Zinc finger protein of 95 kDa and shares homology with transcription factor. Bcl6 is mainly expressed in normal germinal center B cells and related lymphomas. Bcl6 plays an important role in the regulation of the Th2 inflammatory response. Mice deficient in Bcl6 can develop severe Th2-type inflammation in multiple organs. This antibody reacts with Bcl-6 gene product in follicular lymphomas, diffuse large B cell lymphomas, Burkitt's lymphomas and in nodular, lymphocyte predominant Hodgkin's disease.
Primary Antibody
Name: Bcl-6 Antibody |
Clone: P1F6, Mouse Anti-Human |
Supplier: Abcam |
Catalog Number: ab9479 |
Dilution: 1:20 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60min/room temperature |
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102) |
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100) |
Heat/Cool Temperature: 95-100 ºC/room temperature |
Heat/Cool Time: 20 minutes/20 minutes |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Nulcear |
Images: Search image |
Tissue Type: Tonsil, follicular lymphomas |
Fixation: Formalin-fixed paraffin sections, or acetone fixed frozen sections |
Positive Control: Tonsil, follicular lymphomas |
Negative Control: Omit primary antibody, isotype control, absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
There is no need to perform antigen retrieval on acetone fixed frozen sections |
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