Description: Bcl-w belongs to the bcl-2 family of proteins and promotes cell survival, whereas other members such as bak and bax are antagonists and promote apoptosis. The Bcl-w gene is highly conserved between mice and man. Bcl-w protein is found in a diverse range of tissues
including cerebellum, hippocampus, colon, liver, heart, stomach, skeletal muscle, testis and
placenta. It is also expressed in B and T cell lines. Bcl-w is apparently dispensable in normal
development and function of most organs but is essential for spermatogenesis.
Primary Antibody
Name: Bcl-w Antibody |
Clone: 6C1, Mouse Anti-Human |
Supplier: Novocastra Labs |
Catalog Number: NCL-Bcl-W |
Dilution: 1:20 - 1:40 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60min/room temperature |
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102) |
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100) |
Heat/Cool Temperature: 95-100 ºC/room temperature |
Heat/Cool Time: 20 minutes/20 minutes |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Cytoplasmic |
Images: Search image |
Tissue Type: Cerebellum |
Fixation: Formalin-fixed paraffin sections |
Positive Control: Cerebellum |
Negative Control: Omit primary antibody, isotype control, absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
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