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beta-Amyloid Antibody Staining Protocol for Immunohistochemistry
Description: beta-Amyloid protein is 4.2-kD polypeptide. Glenner and Wong (1984)purified a protein derived from the twisted beta-pleated sheet fibrils present in cerebrovascular amyloidoses and in the amyloid plaques associated with Alzheimer disease. The proteins from both disorders have an identical 28-amino acid sequence. memory deficits in middle-aged Tg2576 mice are caused by the extracellular accumulation of a 56-kDa soluble amyloid-beta assembly. Amyloid beta protein (Abeta) deposition in the brain is a hallmark of Alzheimer's disease (AD).
Primary Antibody
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Clone: Mouse monoclonal: AP-5 |
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Supplier: IHC World |
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Catalog Number: IW-MA1109 |
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Dilution: Ready to use |
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Incubation Time/Temp: 60min/room temperature |
Antigen Retrieval
| Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102) |
| Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100) |
| Heat/Cool Temperature: 95-100 ºC/room temperature |
| Heat/Cool Time: 20 minutes/20 minutes |
Detection Methods
| Standard Method: ABC Method or LSAB Method |
| Enhanced Method: Polymeric Methods |
Chromogen Substrate
| Reagent: DAB |
| Incubation Time/Temperature: 1-3 minutes/room temperature |
Counterstain
| Reagent: Mayer's Hematoxylin |
| Staining Time: 30 seconds |
Results:
| Staining Pattern: Extracellular senile plaque and neurofibrillary tangles. |
| Images: Search image |
Additional Information:
| Species Reactivity: Human, mouse. |
| Fixation: Formalin-fixed paraffin embedded tissue sections. |
| Positive Control: Alzheimer’s brain. |
| Negative Control: Omit primary antibody, isotype control, absorption control |
| Blocking: Normal serum blocking is not needed for this RTU antibody; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
References: