beta-Amyloid Antibody Staining Protocol for Immunohistochemistry


Description: beta-Amyloid protein is 4.2-kD polypeptide. Glenner and Wong (1984)purified a protein derived from the twisted beta-pleated sheet fibrils present in cerebrovascular amyloidoses and in the amyloid plaques associated with Alzheimer disease. The proteins from both disorders have an identical 28-amino acid sequence. memory deficits in middle-aged Tg2576 mice are caused by the extracellular accumulation of a 56-kDa soluble amyloid-beta assembly. Amyloid beta protein (Abeta) deposition in the brain is a hallmark of Alzheimer's disease (AD).


Primary Antibody

Name: beta-Amyloid IHC Antibody

Clone: Mouse monoclonal: AP-5

Supplier: IHC World

Catalog Number: IW-MA1109

Dilution: Ready to use

Incubation Time/Temp: 60min/room temperature

Antigen Retrieval
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102)
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100)
Heat/Cool Temperature: 95-100 ºC/room temperature
Heat/Cool Time: 20 minutes/20 minutes

Detection Methods
Standard Method: ABC Method or LSAB Method
Enhanced Method: Polymeric Methods

Chromogen Substrate
Reagent: DAB
Incubation Time/Temperature: 1-3 minutes/room temperature

Reagent: Mayer's Hematoxylin
Staining Time: 30 seconds

Staining Pattern: Extracellular senile plaque and neurofibrillary tangles.
Images: Search image

Additional Information:
Species Reactivity: Human, mouse.
Fixation: Formalin-fixed paraffin embedded tissue sections.
Positive Control: Alzheimer’s brain.
Negative Control: Omit primary antibody, isotype control, absorption control
Blocking: Normal serum blocking is not needed for this RTU antibody; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary