BM1 (Myeloid Marker) Antibody Staining Protocol for Immunohistochemistry


Description: NCL-BM1 is reactive with a 183kD myeloid-specific DNA-binding protein. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. NCL-BM1 and NCL-BM2 react with early precursor and mature forms of human myeloid cells.


Primary Antibody

Name: BM1 (Myeloid Marker) Antibody

Clone: BM-1, Mouse Anti-Human

Supplier: Novocastra Labs

Catalog Number: NCL-BM1

Dilution: 1:20 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed.

Incubation Time/Temp: 60min/room temperature

Antigen Retrieval
Device: Antigen retrieval is not needed
Buffer/pH value: N/A
Heat/Cool Temperature: N/A
Heat/Cool Time: N/A

Detection Methods
Standard Method: ABC Method or LSAB Method
Enhanced Method: Polymeric Methods

Chromogen Substrate
Reagent: DAB
Incubation Time/Temperature: 1-3 minutes/room temperature

Reagent: Mayer's Hematoxylin
Staining Time: 30 seconds

Staining Pattern: Cytoplasmic and nuclear
Images: Search image

Additional Information:
Tissue Type: Granulocytic sarcoma
Fixation: Formalin-fixed paraffin sections, or acetone fixed frozen sections
Positive Control: Granulocytic sarcoma
Negative Control: Omit primary antibody, isotype control, absorption control
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary


There is no need to perform antigen retrieval on acetone fixed frozen sections