Description: NCL-BM2 is reactive with a cytoplasmic antigen expressed in human granulocytes. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. NCL-BM1 and NCL-BM2 are reported to react with early precursor and mature forms of human myeloid cells.
Primary Antibody
Name: BM2 (Myeloid Marker) Antibody |
Clone: BM-2, Mouse Anti-Human |
Supplier: Novocastra Labs |
Catalog Number: NCL-BM2 |
Dilution: 1:25 - 1:50 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60min/room temperature |
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102) |
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100) |
Heat/Cool Temperature: 95-100 ºC/room temperature |
Heat/Cool Time: 20 minutes/20 minutes |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Cytoplasmic |
Images: Search image |
Tissue Type: Blood or bone marrow |
Fixation: Formalin-fixed paraffin sections, or acetone fixed frozen sections |
Positive Control: Blood or bone marrow |
Negative Control: Omit primary antibody, isotype control, absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
There is no need to perform antigen retrieval on acetone fixed frozen sections |
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