Bone Morphogenetic Protein 4 (BMP4) Antibody Staining Protocol for Immunohistochemistry


Description: Bone morphogenetic protein 4 (BMP4) is one of nine structurally related BMPs belonging to the transforming growth factor beta (TGF-beta) superfamily of secreted proteins. Mature BMP4 is a dimer that binds to a multimeric transmembrane receptor with serine/threonine kinase activity. BMP4 has important roles as a signalling molecule in the developing central and peripheral nervous system, musculature and skeleton. It also participates in a signalling pathway found in insects and worms. BMP also plays an important role in early tooth morphogenesis.


Primary Antibody

Name: Bone Morphogenetic Protein 4 Antibody

Clone: 3H2, Mouse Anti-Human

Supplier: Novocastra Labs

Catalog Number: NCL-BMP4

Dilution: 1:20 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed.

Incubation Time/Temp: 60min/room temperature

Antigen Retrieval
Device: Antigen retrieval is not needed
Buffer/pH value: N/A
Heat/Cool Temperature: N/A
Heat/Cool Time: N/A

Detection Methods
Standard Method: ABC Method or LSAB Method
Enhanced Method: Polymeric Methods

Chromogen Substrate
Reagent: DAB
Incubation Time/Temperature: 1-3 minutes/room temperature

Reagent: Mayer's Hematoxylin
Staining Time: 30 seconds

Staining Pattern: Cytoplasmic
Images: Search image

Additional Information:
Tissue Type: Prostatic adenocarcinoma or osteoclastoma
Fixation: Formalin-fixed paraffin sections
Positive Control: Prostatic adenocarcinoma or osteoclastoma
Negative Control: Omit primary antibody, isotype control, absorption control
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary