Bombesin Antibody Staining Protocol for Immunohistochemistry


Description: Gastrin-releasing peptide is a regulatory peptide common for mammalian species: the amphibian counterpart is bombesin. Bombesin is expressed in neural cells and in many neuroendocrine cells.


Primary Antibody

Name: Bombesin Antibody

Clone: Polyclonal, Rabbit Anti-Human

Supplier: Novocastra Labs

Catalog Number: NCL-BOMp

Dilution: 1:200 - 1:400 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed.

Incubation Time/Temp: 60min/room temperature

Antigen Retrieval
Device: Antigen retrieval is not needed
Buffer/pH value: N/A
Heat/Cool Temperature: N/A
Heat/Cool Time: N/A

Detection Methods
Standard Method: ABC Method or LSAB Method
Enhanced Method: Polymeric Methods

Chromogen Substrate
Reagent: DAB
Incubation Time/Temperature: 1-3 minutes/room temperature

Reagent: Mayer's Hematoxylin
Staining Time: 30 seconds

Staining Pattern: Cytoplasmic
Images: Search image

Additional Information:
Tissue Type: Foetal lung
Fixation: Formalin-fixed paraffin sections
Positive Control: Foetal lung
Negative Control: Omit primary antibody, isotype control, absorption control
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary