Description: The c-kit proto-oncogene encodes a transmembrane tyrosine kinase receptor, c-kit (CD117) which is closely related to the platelet derived growth factor family. c-kit plays a role during haematopoiesis, gametogenesis and melanogenesis.
Primary Antibody
Name: c-kit Oncoprotein (CD117) Antibody |
Clone: 57A5D8, Mouse anti-Human |
Supplier: Novocastra Labs |
Catalog Number: NCL-cKIT |
Dilution: 1:200 - 1:400 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60 minutes/room temperature |
Device: No antigen retrieval is needed |
Buffer/pH value: N/A |
Heat/Cool Temperature: N/A |
Heat/Cool Time: N/A |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Membrane/cytoplasmic |
Images: Search image |
Tissue Type: Skin |
Fixation: Acetone fixed frozen sections. |
Positive Control: Normal skin, epidermal melanocytes. |
Negative Control: Omit primary antibody, isotype control or absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
Not tested on paraffin sections |
References: