 |
|
CAK (Cdk-Activating Kinase) Antibody Staining Protocol for Immunohistochemistry
Description: Cyclin dependent kinase (cdk) activation is dependent on cyclin binding and phosphorylation of a conserved threonine residue mediated by cdk-activating kinase (CAK). A cdk-related kinase, MO15, has been identified as the catalytic subunit for CAK. CAK is a protein kinase complex consisting of CDK7 + Cyc H + Mat 1. DNA repair mechanisms and regulation of gene activity both involve CAK.
Primary Antibody
|
Name: CAK Antibody |
|
Clone: MO-1.1, Mouse anti-Human |
|
Supplier: Novocastra Labs |
|
Catalog Number: NCL-CAK |
|
Dilution: 1:50 - 1:100 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
|
Incubation Time/Temp: 60min/room temperature |
Antigen Retrieval
| Device: Antigen retrieval is not needed |
| Buffer/pH value: N/A |
| Heat/Cool Temperature: N/A |
| Heat/Cool Time: N/A |
Detection Methods
| Standard Method: ABC Method or LSAB Method |
| Enhanced Method: Polymeric Methods |
Chromogen Substrate
| Reagent: DAB |
| Incubation Time/Temperature: 1-3 minutes/room temperature |
Counterstain
| Reagent: Mayer's Hematoxylin |
| Staining Time: 30 seconds |
Results:
| Staining Pattern: Nuclear |
| Images: Search image |
Additional Information:
| Tissue Type: Skin or tonsil |
| Fixation: Acetone fixed frozen sections. |
| Positive Control: Skin or tonsil |
| Negative Control: Omit primary antibody, isotype control, absorption control |
| Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
References: