CAK (Cdk-Activating Kinase) Antibody Staining Protocol for Immunohistochemistry


Description: Cyclin dependent kinase (cdk) activation is dependent on cyclin binding and phosphorylation of a conserved threonine residue mediated by cdk-activating kinase (CAK). A cdk-related kinase, MO15, has been identified as the catalytic subunit for CAK. CAK is a protein kinase complex consisting of CDK7 + Cyc H + Mat 1. DNA repair mechanisms and regulation of gene activity both involve CAK.


Primary Antibody

Name: CAK Antibody

Clone: MO-1.1, Mouse anti-Human

Supplier: Novocastra Labs

Catalog Number: NCL-CAK

Dilution: 1:50 - 1:100 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed.

Incubation Time/Temp: 60min/room temperature

Antigen Retrieval
Device: Antigen retrieval is not needed
Buffer/pH value: N/A
Heat/Cool Temperature: N/A
Heat/Cool Time: N/A

Detection Methods
Standard Method: ABC Method or LSAB Method
Enhanced Method: Polymeric Methods

Chromogen Substrate
Reagent: DAB
Incubation Time/Temperature: 1-3 minutes/room temperature

Reagent: Mayer's Hematoxylin
Staining Time: 30 seconds

Staining Pattern: Nuclear
Images: Search image

Additional Information:
Tissue Type: Skin or tonsil
Fixation: Acetone fixed frozen sections.
Positive Control: Skin or tonsil
Negative Control: Omit primary antibody, isotype control, absorption control
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary