Calretinin Antibody Staining Protocol for Immunohistochemistry

Description: Calbindin D28k and calretinin (also designated CR or 29 kDa Calbindin) are two closely related intracellular calcium-binding proteins belonging to the troponin-C superfamily. Initially isolated from chick retina, calretinin shares 58% identical residues with human calbindin D28k. Calretinin is expressed as a 29 kDa protein in brain and is particularly abundant in auditory neurons with precisely timed discharges. Neurons in the nucleus accumbens containing calretinin all possess nuclear indentations. Calretinin-immunoreactive boutons form asymmetrical and symmetrical synaptic specializations on spines, dendrites and somata. The symmetrical synaptic specializations have medium-sized spiny neurons and contact other Calretinin-immunoreactive somata. Calretinin is widely used as a immunocytochemical marker for mesothelioma.