Description:
Calbindin D28k and calretinin (also designated CR or 29 kDa
Calbindin) are two closely related intracellular calcium-binding
proteins belonging to the troponin-C superfamily. Initially isolated
from chick retina, calretinin shares 58% identical residues with
human calbindin D28k. Calretinin is expressed as a 29 kDa protein in
brain and is particularly abundant in auditory neurons with
precisely timed discharges. Neurons in the nucleus accumbens
containing calretinin all possess nuclear indentations.
Calretinin-immunoreactive boutons form asymmetrical and symmetrical
synaptic specializations on spines, dendrites and somata. The
symmetrical synaptic specializations have medium-sized spiny neurons
and contact other Calretinin-immunoreactive somata. Calretinin is
widely used as a immunocytochemical marker for mesothelioma.
Primary Antibody
Name: Calretinin Antibody |
Clone: Goat anti-Calretinin |
Supplier: Santa Cruz Biotechnology |
Catalog Number: sc-11644 |
Dilution: 1:500 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60min/room temperature |
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102) |
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100) |
Heat/Cool Temperature: 95-100 ºC/room temperature |
Heat/Cool Time: 20 minutes/20 minutes |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Cytoplasmic |
Images: Search image |
Tissue Type: Brain (cerebellum) |
Fixation: Formalin fixed paraffin sections |
Positive Control: Brain (cerebellum) |
Negative Control: Omit primary antibody, isotype control, absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
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