Description: Caspases are an extended family of cysteine proteases that play critical roles in apoptosis. Caspases are synthesised as precursors known as pro-caspases and these are converted into mature enzymes by apoptotic signals. Caspase-2 is an early effector in the apoptotic cascade and precedes the activation of caspase-3. It is generally found in the nucleus, but pro-caspase-2 may also be detected in mitochondrial and cytosolic fractions. Lymphoid organs display only weak caspase-2 expression, located in sinusal histiocytes and thymic epithelial cells. Extra-lymphoid caspase-2 reactivity is found in particular organs like the kidney. Bcl-2 oncoprotein may play a central role in inhibiting the caspase-2/caspase-3 cascade.
Primary Antibody
Name: Caspase-2 Antibody |
Clone: 10H2, Mouse anti-Human |
Supplier: Novocastra Labs |
Catalog Number: NCL-CASP-2 |
Dilution: 1:20 - 1:40 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60min/room temperature |
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102) |
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100) |
Heat/Cool Temperature: 95-100 ºC/room temperature |
Heat/Cool Time: 20 minutes/20 minutes |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Nuclear |
Images: Search image |
Tissue Type: Testis |
Fixation: Formalin fixed paraffin sections |
Positive Control: Testis |
Negative Control: Omit primary antibody, isotype control or absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
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