Description: The caspases represent a family of sulphydryl proteases which play important regulatory roles within the cell. Caspase-8, also called FLICE, has an N-terminal domain with sequence homology to the death effector domain of FADD that allows association of caspase-8 with the TNF/Fas family of receptors. This association with the cell surface death receptors has shown caspase-8 to be a proximal regulator of apoptosis. Caspase-8 is activated by association with the Fas/FADD death-inducing signaling complex to release two active subunits, p18 and p10, into the cytosol where they activate other caspases amplifying the apoptotic signal.
Primary Antibody
Name: Caspase-8 Antibody |
Clone: 11B6, Mouse anti-Human |
Supplier: Novocastra Labs |
Catalog Number: NCL-CASP-8 |
Dilution: 1:30 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60min/room temperature |
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102) |
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100) |
Heat/Cool Temperature: 95-100 ºC/room temperature |
Heat/Cool Time: 20 minutes/20 minutes |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Cytoplasmic/Nuclear |
Images: Search image |
Tissue Type: Testis |
Fixation: Formalin fixed paraffin sections, or acetone fixed frozen sections |
Positive Control: Testis |
Negative Control: Omit primary antibody, isotype control or absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
There is no need to perform antigen retrieval for acetone fixed frozen sections |
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