Caspase-8 Antibody Staining Protocol for Immunohistochemistry

Description: The caspases represent a family of sulphydryl proteases which play important regulatory roles within the cell. Caspase-8, also called FLICE, has an N-terminal domain with sequence homology to the death effector domain of FADD that allows association of caspase-8 with the TNF/Fas family of receptors. This association with the cell surface death receptors has shown caspase-8 to be a proximal regulator of apoptosis. Caspase-8 is activated by association with the Fas/FADD death-inducing signaling complex to release two active subunits, p18 and p10, into the cytosol where they activate other caspases amplifying the apoptotic signal.