Catenin γ Antibody Staining Protocol for Immunohistochemistry

 

Description: Catenin γ, also known as junction plakoglobin (JUN) or plakoglobin (PKGB). Plakoglobin is a major cytoplasmic protein which is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. Cateninβ and cateninγ (plakoglobin), vertebrate homologs of Drosophila armadillo, function in cell adhesion and the Wnt signaling pathway. Cateninγ may have distinct roles in Wnt signaling and cancer via differential effects on downstream target genes.

 

Primary Antibody

Name: Catenin γ IHC Antibody

Clone: Rabbit Polyclonal

Supplier: IHC World

Catalog Number: IW-MA1117

Dilution: Ready to Use

Incubation Time/Temp: 60 min/room temperature


Antigen Retrieval
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102)
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100)
Heat/Cool Temperature: 95-100 ºC/room temperature
Heat/Cool Time: 20 minutes/20 minutes

Detection Methods
Standard Method: ABC Method or LSAB Method
Enhanced Method: Polymeric Methods

Chromogen Substrate
Reagent: DAB
Incubation Time/Temperature: 1-3 minutes/room temperature

Counterstain
Reagent: Mayer's Hematoxylin
Staining Time: 30 seconds

Results:
Staining Pattern: Cytoplasmic
Images: Search image

Additional Information:
Species Reactivity: Human, mouse, rat
Fixation: Formalin fixed paraffin embedded sections or cultured cells
Positive Control: Human mammary cancer
Negative Control: Omit primary antibody, isotype control, absorption control
Blocking: Normal serum blocking is not needed for this RTU antibody; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary

 

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