Description: The catenins (α, β, γ, δ) are ubiquitously expressed, cytoplasmic proteins that associate with E-cadherin at cellular junctions. α-catenin also associates with P-cadherin and N-cadherin. β-catenin co-immunoprecipitates with APC. γ-catenin associates with both N-cadherin and E-cadherin and is a major component of desmosomes, where it is found complexed to desmoglein. δ-catenin interacts with Presenilin 1. A hemizygous deletion of the gene encoding δ-catenin correlates with mental retardation in cri-du-chat syndrome. Armadillo Repeat gene deleted in Velo-Cardio-Facial syndrome (ARVCF) is also a member of the catenin family that may function as a nuclear protein.
Primary Antibody
Name: Beta Catenin Antibody |
Clone: Goat anti-beta Catenin |
Supplier: Santa Cruz Biotechnology |
Catalog Number: sc-1496 |
Dilution: 1:200 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60min/room temperature |
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102) |
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100) |
Heat/Cool Temperature: 95-100 ºC/room temperature |
Heat/Cool Time: 20 minutes/20 minutes |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Membrane/Cytoplasmic (can translocated to nucleus under certain conditions) |
Images: Search image |
Tissue Type: Intestine |
Fixation: Formalin fixed paraffin sections |
Positive Control: Intestine |
Negative Control: Omit primary antibody, isotype control or absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
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