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CBFA1/RUNX2 Antibody Staining Protocol for Immunohistochemistry
Description:
Core binding factor A1 (CBFA1/RUNX2) is a
runt-like transcription factor essential for osteoblast
differentiation. This
protein is a member of the RUNX family of transcription factors and
has a Runt DNA-binding domain. It is essential for osteoblastic
differentiation and skeletal morphogenesis and acts as a scaffold
for nucleic acids and regulatory factors involved in skeletal gene
expression. D'Souza
et al. (1999) indicate a non-redundant role for Cbfa
Primary Antibody
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Name: CBFA1/RUNX2 IHC Antibody |
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Clone: Rabbit Polyclonal |
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Supplier: IHC World |
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Catalog Number: IW-PA1224 |
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Dilution: Ready to Use |
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Incubation Time/Temp: 60 min/room temperature |
Antigen Retrieval
| Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102) |
| Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100) |
| Heat/Cool Temperature: 95-100 ºC/room temperature |
| Heat/Cool Time: 20 minutes/20 minutes |
Detection Methods
| Standard Method: ABC Method or LSAB Method |
| Enhanced Method: Polymeric Methods |
Chromogen Substrate
| Reagent: DAB |
| Incubation Time/Temperature: 1-3 minutes/room temperature |
Counterstain
| Reagent: Mayer's Hematoxylin |
| Staining Time: 30 seconds |
Results:
| Staining Pattern: Cytoplasmic |
| Images: Search image |
Additional Information:
| Species Reactivity: Human, mouse, rat |
| Fixation: Formalin fixed paraffin embedded sections |
| Positive Control: Human osteosarcoma |
| Negative Control: Omit primary antibody, isotype control, absorption control |
| Blocking: Normal serum blocking is not needed for this RTU antibody; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
References: