Description: The stem cell factor receptor, c-kit (CD117), acts as an inhibitor of apoptotic cell death upon binding of its ligand. Strong positive immunoreactivity is observed in mast cells of epithelial and hematopoietic tissues. Glial cells from both fetal and adult brain show positive staining, also ductal cells in mammary glands. Tumor cells:c-Kit expression is demonstrated in small cell and large cell lung carcinoma.
Primary Antibody
Name: CD117 (c-Kit) Antibody |
Clone: Rabbit polyclonal |
Supplier: Dako |
Catalog Number: A4502 |
Dilution: 1:200 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60min/room temperature |
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102) |
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100) |
Heat/Cool Temperature: 95-100 ºC/room temperature |
Heat/Cool Time: 20 minutes/20 minutes |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Cytoplasmic |
Images: Search image |
Tissue Type: Human tonsil, intestinal tissues, skin, brain, lung |
Fixation: Formalin fixed paraffin sections, or acetone fixed frozen sections |
Positive Control: Human tonsil, intestinal tissues, skin, brain, lung |
Negative Control: Omit primary antibody, isotype control or absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
There is no need to perform antigen retrieval for acetone fixed frozen sections |
References: